| 1. | When you convert character or binary expressions
将字符或二进制表达式（ |
| 2. | By digesting with bamhi and sacl , we confirmed that the construction of the binary expression vector was correct
选择带有gus报告基因( 1 . 87kb )的pbii21质粒为表达载体，其筛选标记为卡那霉素。 |
| 3. | A binary code in which sequential numbers are represented by binary expressions , each of which differs from the preceding expression in one place only
一种二进制编码，其中顺序的数采用二进制表达式，每一个表达式仅在一位上和它前一个数的表达式不同。 |
| 4. | The next and last udf to cover bitwise operations is bitwise not that performs a bitwise logical not operation for one given integer value as translated to a binary expression
下一个也是最后一个涉及逐位运算的udf是逐位not运算，在转换成二进制表达式时，该udf对给定的整数值执行逐位逻辑not运算。 |
| 5. | 3 . arabidopsis plants were transformed with the constructed plant binary expression vector . eighteen independent antibiotic - resistant arabidopsis transformants for dreb1c were obtained by using a vacuum infiltration method
载体全长为13kb ，有一个烟草花叶病毒35s强启动子和nos终止序列，利用bamhi和sacl做为酶切位点，构建载体。 |
| 6. | By blue - white screening and sequence analysis , we obtained the positive clone . 2 . we constructed plant binary expression vector carrying fusions of the enhanced camv 35s promoter and dreb1c ( 35s : dreb1c ) in the sense orientation
我们从拟南芥基因组中克隆了dreb1c基因全长，并将其连接到中间载体pgem - t - easy上，进行了测序验证，结果显示所克隆的dreb1c序列完全正确。 |
| 7. | Construction of plant binary expression vector peasp in consideration of the different cell membrane disruption mechanism of plant defensin and cema , plant binary expression vector peasp were constructed . simultaneous expression of cema and afp may synergistically inhibit the growth of phytopathogens that are the main cause agent of agricultural loss
构建了双价抗菌肽基因植物表达载体peasp鉴于植物防御素与cema的空间结构、作用机制有所不同，两者在植物中的同时表达，有可能对病源真菌有协同抑制作用，构建了双价植物表达载体peasp 。 |
| 8. | Three binary expression vectors were constructed , which harbored the cl - i - 2kl , r - 2kl , and d - l - 2kl - r - 2kl respectively , driven by the camv 35s promoter . then , agrofeac / erium - mediated transformation of iobacco ( nicotiana tabacum , w38 ) was carried out . transformed tobacco lines were obtained through tissue culture and confirmed by pcr and southern blotting analysis
通过分子克隆技术，将c1 - i - 2k1 、 r - 2k1和二者的连接( c1 - i - 2k1 ? r - 2k1 )分别构建到双元表达载体上，并使它们分别置于35s启动子的驱动之下，然后通过农杆菌介导的技术分别对烟草( nicotianatabacum ， w38 )进行了转化，利用组织培养技术再生植株。 |
| 9. | The plant expression vector pbin19 - rok219 was constructed , which contains cauliflower mosaic virus ( camv ) 35s promoter and noster . introduction of ibdv vp2 gene under control of camv 35s promoter resulted in the construction of binary expression vector pbr - vp2 . then the vp2 gene was in the left and right border regions , which denote the limits of the dna that is integrated into the plant genomic dna via agrobacterium fwme / ac / ms - mediated transformation
构建了表达载体pbin19 - rok219 ，以ibdv甘肃天水株的抗原基因vp2为目的基因，将vp2基因置于植物组成型表达启动子camv35s之下，构建了一个ibdvvp2基因的植物表达载体pbr - vp2 ，这样使vp2基因位于农杆菌t - dna的左右边界重复序列之间。 |
